Group&vacancies
Stage / Internships
Multiple positions are available:
Lipid second messengers in plant growth and development
Phosphatidic acid (PA) and polyphosphoinositides (PPIs) are minor phospholipids in biological membranes, functioning as signalling molecules at verylow concentrations. Their turnover is much faster than that of structural phospholipids and levels quickly change in response to various biotic-(pathogens) and abiotic- (salt, cold, heat) stresses. How this is regulated and where this occurs within the cell (e.g. plasma membrane, ER, Golgi) and whole plant (e.g. root, stem, stomata) is mostly unknown. In the genome of the model plant system Arabidopsis thaliana, various lipid kinases, phosphatases and phospholipases have been identified. Using T-DNA insertion knock-out mutants, we are pinpointing their individual contribution in stress and plant development. Using specific lipid-binding domains,fused to a fluorescent protein and stably expressed in cultured cells and whole plants, we can visualize where these lipid-second messengers are present in living cells. Students can be involved in any of the projects.
Techniques:
* Mutant physiology (biotic/abiotic stress)
* Radiolabeling & Lipid biochemistry
* Molecular cloning strategies
* RNA/DNA (e.g. PCR/Northern analysis)
* Protein extraction & analysis (Western/ MS)
* Confocal Laser Scanning Microscopy (CLSM)
Contact:
Teun Munnik
Stagecoördinator section Plant Physiology
Swammerdam Institute for Life Sciences (SILS)
University of Amsterdam (UvA)
Science Park 904,
1098 XH, Amsterdam
The Netherlands
Tel: +31 (0)20 525 7763
Research
Phospholipid Signalling Group
Teun Munnik (PI)
Xavier Zarza (PhD student, Barcelona University)
Laura Zonia (post-doc)
Aleksandra 'Ola' Haduch (PhD student, Poland)
Ringo van Wijk (technician)
Wendy Roels (technician)
Jiorgos Kourelis (student)
Carlos Jr. Rubio 'Chucho' (student)
Christa Testerink (PI)
Fionn McLoughlin (PhD student)
Carlos Galvan (post-doc)
Ex-group members
Dörte Klaus (Toulouse, F)
Christiane Unger (Germany)
Alan Musgrave (retired)
Michael Miskind (NSF, VA, USA) Sabbatical
Ana Laxalt (Mar del Plata, Argentina)
Bas ter Riet (NKI, Amsterdam, NL)
Harold Meijer (WUR, Wageningen, NL)
Christa Testerink (SILS, UVA)
Wessel van Leeuwen (WUR, Wageningen, NL)
Rafa Tobeña (Madrid, Spain)
Gert-Jan de Boer (Enza Zaden, Enkhuizen, NL)
John van Himbergen (VROM)
Diewertje van der Does(UU, Utrecht, NL)
Saskia van Wees (UU, Utrecht, NL)
Bastiaan Bargmann (NY, USA)
Fabio Formiggini (Italy)
Gaby Gonorazky (Mar del Plata, Argentina)
Martine den Hartog (Covidien, Amsterdam)
Arnold van der Luit (Oncodesign, France)
Bastiaan Bargmann (NY, USA)
Bas van Schooten (UC, CA, USA)
Essam Darwish (Cairo, Egypt)
Joop Vermeer (Lausanne, Switzerland)
Steven Arisz (UvA, Amsterdam)
Muhammad Shahbaz (Faisalabad, Pakistan)
ex-undergraduate students
Wladimir Tameling
Martine den Hartog
Julian C. Verdonk
Steven Arisz
Bas ter Riet
Elske Schouten
Diewertje van der Does
Monique Raats
Tamara Chessa
Jacco van Rheenen
Nathalie Verhoef
Tessa Nauta
Thanh Le
Collaborating Labs
Dorus Gadella (SILS, UvA, NL)
Nullin Divecha (Manchester, UK)
Mats Ellerström (Göteborg University, Sweden)
Takashi Aoyama (Kyoto University, Japan)
Ana Laxalt (University of Mar del Plata, Argentina)
Matthieu Joosten (WUR, Wageningen, NL)
Charles Brearley (Norwich, UK)
Chris de Koster (SILS, UvA, NL)
Robin Irvine (Cambridge, UK)
Laura de la Canal (Mar del Plata, Argentina)
Nick Ktistakis (Cambridge, UK)
RalfOelmüller (Jena, Germany)
Heribert Hirt (Vienna, Austria)
Dierk Scheel (Halle, Germany)
Dorothea Bartels (Bonn, Germany)
George Carman (Rutgers, NJ, US)
Laci Bögre (London, UK)
Susanne Hoffmann-Benning (Michigan, US)
Pavla Binarova (Prague, Czech)
Ingo Heilmann (Halle, Germany)
Jörg Kudla (Münster, Germany)
Silke Robatzek (Norwich, UK)
Mariusz Pietruszka (University of Silesia, Poland)
Antonio Fernandez Tiburcio (Barcelona, Spain)